WebCYTEK Aurora General Operation Protocol Sabrina Förstner update 17/03/2024_SP 6 6) Select (+) Reference Groups for unmixing NOTE: If you plan to unmix the samples using only reference controls run in QC & Setup, this step is not necessary. 7) IMPORTANT: Define an unstained control for autofluorescence by selecting its control type (beads or … WebThe gains pre-loaded in the software are derived from the “Cytek Assay Settings” which are based on the QC beads and are calculated on a daily basis during the quality control process. Cytek Assay Settings are a universal baseline for instrument performance to facilitate stability and normalization across instruments and time.
Preparing a Spectroflo Experiment
WebAug 21, 2024 · Cytek ® Aurora; Cytek ® Aurora CS; Cytek ® Northern Lights ™ Cytek ® NL-CLC; Reagents keyboard_arrow_up. cFluor ® Reagents; Tonbo ™ Reagents; … WebUse ZE-Series QC Beads to check that the optics and fluidics the ZE5 Cell Analyzer are performing to specification. Quality control beads for flow cytometry (3–3.4 μm diameters) Fluorescent dyes emitting from ultraviolet to far red. Mixture of single-peak beads matching all fluorescence channels of the ZE5 Cell Analyzer. iot in robotics
Section 1 - PRODUCT AND COMPANY IDENTIFICATION
WebCytek Biosciences Page 5 of 6 • Adjusting Compensation: Learn how to adjust the spillover matrix and how to copy and paste the matrix to other tubes. • Sharing User Settings & Worksheets: Learn how to share user settings and worksheets across users. • Merge FCS Files: Learn how to merge multiple FCS files into one. Extra Tools • Extra Tools Menu: … WebSetting Up an Experiment on the Cytek Aurora Emory Pediatrics/Winship Flow Cytometry Core Version: 1.02 Updated: 22 March 2024 i. If you will be running some single stained controls on beads and others on cells, check the ‘Define Additional Negative ontrols’ box and add an additional negative control and choose its type. ii. WebThis product contains 3.0 um particles with one fluorescent intensity. Every particle contains a mixture of fluorophores that enables the particles to be excited at any wavelength from 355 to 650nm Cytek® SpectroFlo QC … onward full movie english