WebThe two lipases exhibited moderate activation in the presence of nonionic detergents when used below their CMC values. Both lipases were found to exhibit strong tendency to aggregate as observed through gel filtration ... suggesting that, enzyme aggregation might be a major contributor to the high thermostability of LipA and LipB. Keywords ... WebDetergents with higher CMC values have weaker binding and are subsequently easier to remove by dialysis or displacement methods. Detergents with low CMC values require less detergent in order to form micelles and solubilize proteins or lipids.

Detergents - Anionic, Cationic, Zwitterionic, Anti-foaming

WebMay 1, 2007 · detergent with a high CMC is clearly preferred. Alternatively, if ion . exchange chromatography is utilized, a non-ionic detergent or a ZWITTER-GENT® Detergent is the detergent of choice. 4. Weblower the CMC, the more stable the micelle and the more slowly molecules are incorporated into or removed from the micelle. CMC values are a guide to detergent hydrophobic … diagnostic ankle arthroscopy cpt code

Phase separation in the isolation and purification of

WebDetergents with high CMC can be read-ily removed from protein-detergent complexes by dialysis, whereas low CMC detergents dialyze away very slowly. Membrane with Bound Detergent WebCMC is used as a viscosity modifier or thickener, and to stabilize emulsions in various products, both food and non-food. It is used primarily because it has high viscosity, is nontoxic, and is generally considered to be … WebThe CMC value is 6-10 mM. Detergents with high CMC values are generally easy to remove by dilution; detergents with low CMC values are advantageous for separations on the basis of molecular weight. As a general rule, detergents should be used at their CMC and at a detergent-to-protein weight ratio of approximately ten. diagnostic application of enzymes